Glass pipettes are commonly used in laboratories for transferring liquids and chemicals. However, they can easily become contaminated with residue and impurities, which can affect the accuracy of your experiments. Therefore, it is crucial to clean glass pipettes properly to ensure their longevity and accuracy. In this article, we will discuss the best practices for cleaning glass pipettes.
Step 1: Disassemble the Pipette
Before cleaning the pipette, it is essential to disassemble it. This will allow you to clean each part thoroughly and prevent any residue from being left behind. Carefully remove the rubber bulb or pipette filler from the pipette and set it aside.
Step 2: Rinse with Water
The first step in cleaning a glass pipette is to rinse it with water. Hold the pipette under running water and allow the water to flow through it for a few seconds. This will remove any loose debris or residue.
Step 3: Soak in Detergent Solution
After rinsing the pipette, soak it in a detergent solution. Use a mild detergent that is specifically designed for laboratory glassware. Fill a beaker or container with the detergent solution and place the pipette in it. Allow the pipette to soak for at least 30 minutes.
Step 4: Clean the Pipette
After soaking the pipette, use a pipette brush to clean the inside of the pipette. Gently scrub the inside of the pipette with the brush, making sure to reach all the way to the bottom. Rinse the pipette with water to remove any remaining detergent.
Step 5: Rinse with Deionized Water
To ensure that all detergent residue is removed, rinse the pipette with deionized water. Deionized water is free of impurities and minerals, which can leave residue on the pipette. Rinse the pipette thoroughly with deionized water.
Step 6: Dry the Pipette
After rinsing the pipette, shake off any excess water and allow it to air dry. Do not use a cloth or paper towel to dry the pipette, as this can leave behind fibers or lint.